Introduction Crystal violet staining is a fundamental laboratory technique in microbiology used to differentiate bacterial species based on the structural differences in their cell walls. It is an integral component of the Gram staining method, first developed by Hans Christian Gram in […]
Introduction Subgingival biofilm refers to the structured microbial community that forms below the gumline (gingiva), primarily within the gingival sulcus or periodontal pocket. It plays a critical role in the initiation and progression of periodontal diseases, including gingivitis and periodontitis. Unlike supragingival […]
Introduction Gram-positive bacteria represent a major group of prokaryotic microorganisms characterized by their thick peptidoglycan cell walls that retain the crystal violet stain during the Gram-staining procedure. These bacteria include numerous clinically significant species responsible for a wide range of human infections, […]
Introduction Actinomyces viscosus is a gram-positive, facultative anaerobic, non-motile, and non-spore-forming bacterium that is part of the normal flora of the human oral cavity. It plays a significant role in dental plaque formation and has been implicated in a range of periodontal […]
Introduction Actinomyces israelii is a Gram-positive, non-spore-forming, filamentous, facultatively anaerobic bacterium that belongs to the genus Actinomyces, family Actinomycetaceae. First identified in the late 19th century by James Israel, this bacterium is now recognized as the primary causative agent of actinomycosis, a […]
Introduction Actinomyces naeslundii is a Gram-positive, non-spore-forming, facultatively anaerobic bacterium that is a key member of the human oral microbiome. It was first described by Hamada in 1970 and is part of the genus Actinomyces, which includes other medically relevant species such […]
Introduction Actinomyces is a genus of Gram-positive, facultatively anaerobic or strictly anaerobic, filamentous bacteria known for their role in chronic infections, especially actinomycosis. These organisms are part of the normal flora of the human oropharynx, gastrointestinal tract, and urogenital tract but can […]
Introduction Streptococcus mutans is a Gram-positive, facultative anaerobic bacterium that is widely recognized for its crucial role in the etiology of dental caries. As a prominent member of the oral microbiota, S. mutans is known for its ability to metabolize carbohydrates, produce […]
Introduction Biofilms are structured communities of microorganisms that attach to surfaces and are enclosed in a self-produced extracellular polymeric substance (EPS) matrix. Biofilm formation is a universal microbial survival strategy found in natural, industrial, and clinical environments. From persistent infections to dental […]
Introduction Case-control studies are a cornerstone of observational epidemiology, especially in the investigation of rare diseases or diseases with a long latency period. By comparing individuals with a specific outcome (cases) to those without it (controls), researchers can identify and quantify associations […]